Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

Relationships between the results of hypo-osmotic swelling tests, sperm motility, and fertility in Estonian Holstein dairy bulls

As an attempt to find an inexpensive and simple laboratory method for artificial insemination (AI) bull semen quality assessment, the osmotic resistance of spermatozoa was measured using the hypoosmotic swelling (HOS) test, developed by Jeyendran et al. (1984) (labelled HOS-1), and its modifications (HOS-2, HOS-3), with decreased osmotic pressure aimed at challenging sperm survival ability. The...

Correlation between Different Patterns of Hypo-Osmotic Swelling and Sperm Functional Tests

Correlation between Different Patterns of Hypo-Osmotic Swelling and Sperm Functional Tests

BACKGROUND Sperm membrane integrity is not only important as a barrier between intra- and extra-cellular spaces, but also it can be considered as a sign of DNA integrity. Hypoosmotic swelling test reflects membrane integrity and has been used to evaluate sperm quality. Intracytoplasmic sperm injection (ICSI) in adjunct with hypo-osmotic swelling test (HOST) has been used for treatment of males ...

correlation between different patterns of hypo-osmotic swelling and sperm functional tests

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Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?

Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-1...